ANTIBACTERIAL ACTIVITIES OF CINNAMON ZELANICUM SYZYGIUM AROMATICUM ESSENTIAL OIL

HASSAN A.HUSSEIN1 IBRAHIM S.ABAAS2 RAGHAD HUSSAIN ALI3
1Department of Clinical Laboratory Sciences, College of Pharmacy, University of Kerbala, Iraq, 2Department of pharmacognosy and Medicinal Plants, College of Pharmacy, University of Kerbela, Iraq, 3Emergency Operations, MedicalImam Al-Hussein Medical City,
Kerbala, Iraq.
Email: Raghadhussain35@yahoo.com

ABSTRACT
Objective: Evalution the in vitro antibacterial activity of the essential oil isolated fromCinnamomum zeylanicum bark, Syzygium aromaticum flowers, against Gram-positive (Staphyllococcus aureus, Streptococcus pyogenes) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Serratia marcescens, Enterobacter cloacae, Klebsiella pneumonia ). Methods: Extracts from the Cinnamomum zeylanicum bark, Syzygium aromaticum flowers were hydro distilled for 3 hours in Clevenger’s glass apparatus. 50 ml of diethyl ether was added into 20 gm of chopped spices and the mixture was left for 6 hours, the ethereal layer was dried over
anhydrous sodium sulphate, and ether distilled off on a gently heated water bath, after which the essential oils extracted were collected in amber closed vials and is used, antibacterial screening was carried out by disc diffusion method.
Results: The MIC of the extracts and isolated compounds was determined by broth dilution method, the Cinnamomum zeylanicumwas antibacterial activity on( Staphyllococcus aureus, Streptococcus pyogenes and (Escherichia coli,, Serratia marcescens, Enterobacter cloacae, Klebsiella pneumonia)with inhibition zone (30 mm), while both (Pseudomonas aeruginosa, Proteus mirabilis )were with inhibition zone (28mm).Clove oil showed significant inhibitory effect against Staphyllococcus aureus(20 mm), Streptococcus pyogenes(23 mm) and (Escherichia coli (25 mm), Pseudomonas aeruginosa(16 mm), Proteus mirabilis(23 mm), Serratia marcescens (20 mm), Enterobacter cloacae (25 mm), Klebsiella pneumonia(20mm).the MIC results of the Cinnamomum zeylanicumSyzygium aromaticum, essential oil were indicated that antibacterial activities in lower concentrations 1/128 on Staphyllococcus aureus, Streptococcus pyogenes, Proteus mirabilis Pseudomonas aeruginosa and 1/256 on serratia marcescens, enterobacter cloacae, Escherichia coli, klebsiella pneumonia .
Conclusion; This study was proved that both essential oil were important as herbal drug to used in pharmaceutical industries to treatment infectious diseases.