Investigation of IL6, IL10, IFN-γ and TNF-α at Iraqi patients with colon cancer suffering type-2 diabetes

Hassan A Al-Saadi*
Department of Clinical Laboratory Sciences/Pharmacy College/KerbalaUniversity/Kerbala/Iraq.

ABSTRACT
Objective: Diabetes mellitus is one of the risk factor for all cause of cancer such as colorectal cancer most common among females worldwide. Aimed to investigate the serum levels of IL-6, IL-10 andIFN-γ, TNF-α in patients with colon cancer suffering diabetes mellitus. Materials and methods: Fifty patients with colon cancer (21 female and 29 male) with ages ranged between (20-70) years were taken from (Al-Hussain Hospital City/Kerbala, Digestive and Liver Disease /Education Hospital Medical City Baghdad and Teaching Oncology Hospital /Baghdad Medical City/ Baghdad /Iraq). Compared with 20 healthy persons. Estimation of CEA, Ca19-9 by Vidas, soTNF-α, IFN-γ, IL-6 and IL-10 were studied using the enzyme-linked immunosorbent assay (ELISA) method.
Results: Thirty patients among fifty patients of colon cancer (60%) of patients showed a high diabetes mellitus, fasting plasma glucose was ≥ 126mg/dl.Twenty non-diabetic patients of colon cancer were (40%),observed a significant (p<0.05) increase when comparing the levels of IL-6 and TNF-α in diabetic age group (40-59) yrs with age group (60-70)yrs. In the present study, the results revealed a significant (p<0.05) decrease in the level of IFN-γ when compared no diabetic age group (40-59) with diabetic age group (60-70) yrs.
Conclusion: Elevated (CEA ,Ca 19-9 , IL-6, IL-10, IFN-γ and TNF-α )levels were observed in patients with colon cancer that suffering type 2 diabetes , those patients have high risk of developing diabetes ,these tumor markers and cytokines have a role in diagnosisinterpretation this puzzle in patients.