ABSTRACT : The systemic lupus erythematosus (SLE) is an unexplained etiological inflammatory disorder and a chronic
systematic illness. The aim of the study was the estimation of ANA, ds- DNA, C3, C4, IL-4, IL-10, IL-18, and IL-35 in Systemic Lupus erythematosus patients in Kerbala province. Blood sample (5ml) was collected During the period from 2/October for 31/ December /2018, Using ANA ELISA, Anti-dsDNA ELISA kits, C3, C4 Radial Immunodiffusion Plate, IL-4, IL-10, IL-18 and IL- 35 ELISA kits, 50 patients diagnosed with Systemic Lupus Erythematosus by female clinicians aged 10-70 years. Parameter levels were elevated; ANA, anti-dsDNA, IL-4, IL-10, IL-18 and IL-35 measured in SLE patients reflecting severity. Our results were revealed that ANA optimal values were at age group 21-30 yrs, ds-DNA optimal values at age groups 10-20 yrs and 21-30 yrs, C3, C4 increased values were at age group 61-70 yrs. In all age groups IL-4, IL-10 and IL-18 increased while IL-35 values were decreased in all age groups except age group (10-20). In conclusion, this study suggested the critical role of Interleukins (4,10,18 and 35) inactivation and the regulation of immune responses in patients suffering SLE.IL-4 and immunoregulatory IL- 10 contribute to B cell activation. IL-18 as a biomarker in distinguishing SLE in prognosis early indicator SLE development in lupus nephritis.IL-35 as biomarker detector for SLE activity. These indicators provide us renal disorder impression of SLE.
Key words : Autoimmune disease, IL-4, IL-10, IL18, IL-35, Systemic lupus erythematosus.
