ABSTRACT : Antibiotic resistant bacteria can be considered as a main problem in infection management. Ceftriaxone is a type
of antibiotic that is commonly used to treat the most dangerous bacterial infections. Reducing drug consumption by converting
regular drug forms into nanostructured forms is one strategy to lessen pharmaceutical adverse effects. The aim of the present
study is to preparation ZnO-Ceftriaxone nanoparticles and characterization it by FTIR, XRD, SEM, AFM and EDX, also
determine the antibacterial activity against some Gram negative bacteria Pseudomonas aeruginosa, Proteus mirabilis, klebsiella
pneumonia, Acinetobacter baumannii and Escherichia coli. This can be considered as good candidates for struggling against
drug resistant bacteria. ZnO nanosheets was characterized using FTIR, XRD and SEM while the end product (ZnO-Ceftriaxone
NPs) was fully characterized using FTIR, XRD, SEM and EDX. ZnO-Ceftriaxone NPs was successfully synthesized through
direct ion exchange and characterized, then the efficacy of the antimicrobial activity on eliminating gram-negative bacteria
investigated. The results prove that the novel nanohybrid was successful synthesized with a particle size in the range of
nanoscience. It also showed that Acinetobacter baumannii was most affected by the inhibitory activity of the ZnO-Cefrtiaxone
NPs compared to other bacterial isolates because it was given the highest inhibition zone as it was (24,21,19,15) mm for the
concentrations (16384, 8192, 4096, 2048) μg/ml followed by the bacterium E. coli that gave the lowest inhibition zone as it was
(13, 10, 9, 0) mm for the same concentrations, respectively. But another bacteria Pseudomonas aeruginosa, Proteus mirabilis
and Klebsiella pneumonia seemed to be more resistant to ZnO-Ceftriaxone NPs. In conclusion, results confirm that (ZnOCeftriaxone
NPs) structure is an appropriate antibiotic for treatment infections occurs by Acinetobacter baumannii and
Escherichia coli.
Key words : Ceftriaxone sodium, antibacterial, ZnO-ceftriaxone nanoparticles, Gram negative bacteria.
