ABSTRACT
Background: Osteoporosis, demonstrated as an associated disease with more than 30
gene disorders, is a polygenic disorder, and it’s also implicated in bone mineral density
(BMD) regulations. Lipid peroxidation and hydrogen peroxide levels significantly increased
and vice versa the antioxidant enzymes decreased such as Glutathione S-Reductase
GSR was found in female postmenopausal females. Objective: This research was done
in order to find out the effect of rs2978663 genotypes on the progression of osteoporosis.
Methods: First, blood samples were used to extract DNA for analysis. Molecular
examination was achieved using PCR, RFLP, and UV imaging after electrophoresis in an
agarose gel, and these results were analyzed by SPSS (version 23). Results: The genotypes
differed in healthy people, and the proportions varied, as they were: the highest
percentage was represented by the GA genotype (78%), followed by the AA genotype
(16%), and the GG genotype (6%). For case samples, the highest percentage was represented
by the GA genotype (51%), followed by the AA genotype (30%), and the GG
genotype (19%). There are significant associations between GA genotype and restriction
of fragility disease. The risk of having osteoporosis was significantly lower in those with
the GA genotype (OR = 0.1946; 95% CI = 0.04-0.95; P = 0.03). The A allele frequency
of the GSR gene (rs2978663) did not change significantly between study groups (OR:
0.9965, 95% CI: 0.5547-1.7816, P value: 0.9905). Conclusion: Overall, it is safe to say
that GSR-int3 (rs-2678663) was shown to have no association with osteoporosis in this
research of Iraqi women. Inherent variation in the GSR gene (rs-2678663) is associated
with decreased osteoporosis risk.
Keywords: RFLP-PCR techniques, re2978663, GSR gene polymorphism, ApaLI; SNP.
