Oxidative responses and genetic stability of date palm Phoenix
dactylifera L. Barhi cv. under salinity stress.
Aqeel A.Suhim1* Karima F. Abbas2* Khearallah M.A. Al-Jabary1*
1* Date palm Research Centre- Basra University
2* Environmental Health Dept.- Applied Medical Sciences College – Kerbala University
* E-mail of the corresponding author: karima.f.abbas@gmail.com
Abstract
This study was aimed to investigate the oxidative responses and genetic stability of date palm Phoenix
dactylifera L. under different irrigation water salinity, date palm off shoots cv. Barhi subjected to different
concentrations of NaCl (100, 200, 300 and 400 μM) for 180 days. The obtained results showed that, the date
palm responses to salinity stress, this responses was increased of H2O2 level, Peroxidase activity and
Malondialdehyde MDA concentration with increase NaCl concentration, while opposite trend with membrane
stability index, which H2O2 was increased from(0.73 μM/g) in control to (2.20 μM/g) in 400 μM treatment,
MDA was increased from (2.35 nmole/g) in control to (nmole/g) in 400 μM treatment, also peroxidase activity
was reached to (39.59 U/min/g) in 400 μM treatment, while was (20.73 U/min/g) in control and Membrane
stability index reduction significantly from (81.36%) in control to (64.13%) in 400 μM treatment. In terms of
genetic stability of date palm under salinity stress, the ISSR markers analysis showed that, the high
concentrations of NaCl (200, 300 and 400 μM) produced more polymorphic fragments comparison to control
treatment, while the DNA profile was identical between control and 100 μMtreatment.Dendogram was generated
using similarity indices of ISSR markers showed, the lowest genetic similarity was found between 400 μM NaCl
concentration and control and 100 μM treatment, followed with both 200 and 300 μM treatments, which the
control and 100 μM treatment was grouped in one cluster, also treatments with 200 and 300 μM grouped in one
cluster, while the treatment with 400 μM NaCl separated in cluster.
Keywords: ISSR, Genetic stability, Oxidative stress, Peroxidase, MDA, membrane peroxidation
