Isolation of various local Bacteriophages via Simple Methods and
their Effects against Multidrug Resistance Bacterial Isolates
Suhad Hadi Mohammed, Maysaa Salih Mahdi, Arwa Fadil Haqi, Alaa Haider
Jawad, Ibtihal Dakheel Mohammad, Mays Ahmed Abid Al-Rassol , Noor Yahya
Abid Zaid .
University of Kerbala , Collage of Applied Medical Sciences , Department of
Clinical Laboratories.
Abstract:
Recently, dissemination of antibiotic resistant bacteria considered as a major public
health concern. Therefore, bacteriophages gained great attention as a promising
alternative therapy for resistant infectious diseases.
The current study aimed to isolate bacteriophages by simple methods and determining
their efficiency against bacteria isolated from clinical samples.
Bacteria were first isolated from clinical specimens and identified using standard
bacteriological and biochemical procedures. antibiogram of the isolated bacteria were
determined using different antibiotic discs and the results were interpreted according
to the clinical laboratory standards institute (CLSI) guidelines. Then sewage samples
were processed using two protocols to isolate specific bacteriophages. Finally,
antibacterial effect of bacteriophages was determined using Double Layer Agar(DLA)
method.
