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Summary
The present study includes the identification of parasites, bacteria and viruses responsible for diarrheal infection in children under six years old suffering from acute diarrhea during the period from begning December 2020 till the end of May 2021,they were attending to Babylon teaching Hospital for Maternity and Children in the babylon city.
A total of 320 stool samples from both genders have been collected from the hospitalized patients to identifiy the causative agent of acute diarrhea, the primary detection of parasitic infection was carried out using microscopic and immunochromatography test ( triage Rapid test) as well as Multiplex-PCR which used to detect three parasites infection by used (EHCP8-S1,GLCP6-S1and CRY18s-S1) genes. The Conventional PCR that used to identify bacteria were carried out to detect (pho) gene family for E.coli and 16srRNA gene for Salmonella spp. On the other hand , among the viruses that causes diarrhea the results found Rotavirus infection was carried out using immunochromatography test
(Rapid test) with Reverse Transcriptase-PCR assay used to detect virus infection by using structural gene (vp