Assistant Professor Dr. Alaa Abdul Hussain Kareem, a lecturer in the Department of Pathological Analysis at our College, obtained a certificate from a scientific resident in the Journal of Food Biochemistry, which is affiliated to Wiley. This journal is included in the magazines of Scopus and Citescores by 1.37.

The post Certificate of Evaluation in Scopus International Magazine first appeared on Applied Medical Sciences.

Alaa Abdul Hussein Al-Daamy 1 , Alyaa Ahmed 2 and Ghadeer Mohammad 3
1, 2,3 Department of Clinical laboratories, College of Applied Medical Sciences, University of Kerbala, Kerbala, Iraq.

ABSTRACT

Objective: The current study aimed to screen of local fungi isolates for antimicrobial bioactive compounds production. Methods: Fifteen samples of whisperers soil were analyzed for antimicrobial producing by fungi. Seven species of fungi were obtained from these samples, these fungi were Aspergillus niger, Aspergillus ochraceus, Aspergillus flavus, Penicillium notatum, Bacillomyces sp., Trichoderma sp. and Cladosporium sp. and the filtrate of each fungal isolate given special label as AA, AB, AC, AD, AE, AF and AG, respectively. These fungal filtrates were tested their antimicrobial activity against three Gram positive bacteria (Staphylococcus sp. , Streptococcus agalicae and Bacillus sp.) also three Gram negative bacteria (Enterobacter sp. , Pseudomonas sp. and Klebsiella sp.) by disc technique. Results: Bacillus sp. was the most sensitive to all fungal filtrations, especially for AC (Aspergillus flavus) and AE (Bacillomyces sp.) where the inhibition zone were the largest compared to the antibiotic which used, the inhibition zones were (6 and 5.33)mm, respectively. Streptococcus agalicae was affected only by AD (Penicillinum notatum) and AG (Cladosporium sp.) with inhibition zone ( 4 and 3.33)mm, respectively. In Gram negative bacteria, Enterobacter sp. is highly sensitive to AG (Cladosporium sp.) with inhibition zone 7 mm. Conclusion: Some species of fungi which have antibacterial activity can be isolated from the soil of whisperers.

[/mhc_text][/mhc_column][/mhc_row][/mhc_section]

The post Antimicrobial agents production by fungi isolates from the whisperers first appeared on Applied Medical Sciences.

Alaa Abdul Hussein Al- Daamy 1 , Hassan Kareem Wadi 1 , Rafal Ahmed Abed Ali 1 and Retaj Haider Tauma1
1 Clinical Laboratory Department, Applied Medical Sciences College, Karbala University, Iraq.

ABSTRACT

Objective: The current study aims to investigate of the relationship between dermatophytoses and blood groups in men. Methods: 37 men patients with dermatophytoses who visited the hospitals in the holy province of Karbala were diagnosed with the clinical manifestations of them by dermatologists specialists in these hospitals, and blood samples were obtained in the laboratory and the ABO examination for them to know their blood groups and Rh. Results: Patients are men aged 3 to 80 years. The age group 16-28 years is the highest among the patients, reaching 62.16%. All patients were Rh positive (Rh+). People with blood group O are the most likely to develop dermatophytoses, with 43.23% of patients with blood group B 32.43% and A 16.22% , while the blood group AB is the lowest and 8.11%. The clinical manifestations of dermatophytoses were the most common among the others. The number of patients was 16 patients with tinea pedis most of them from blood groups B and O, followed by tinea capitis which reached 9 patients most of them blood group O. The statistical analysis showed that tinea pedis was significantly correlated with blood groups B and O , and tinea capitis significantly correlated with blood group O, and the rest of the clinical manifestations showed no significant correlation with any of the blood groups. Conclusion: That there is a relationship between infection of dermatophytoses and blood groups, specifically between tinea pedis, tinea capitis with blood groups B and O.

The post The Relationship Between Blood Groups And Dermatophytoses Of Men In Karbala City Alaa Abdul Hussein Al- Daamy 1 , Hassan Kareem Wadi 1 , Rafal Ahmed Abed Ali 1 and Retaj Haider Tauma1 1 Clinical Laboratory Department, Applied Medical Sciences College, Karbala University, Iraq. first appeared on Applied Medical Sciences.

Alaa Abdul Hussein Kareem Al-Daamy,a Ali Abdul Hassan,a Ali Mahmooda

Objectives: The present study aims to determine antibacterial activity for Lawsonia inermis leaf extract against some pathogenic bacteria.
Methods: Five solvents used are acetone, ethanol, methanol, ethyl acetate and distilled water to obtain of crude extract of Lawsonia inermis leaves, which tested the effectiveness on five types of bacteria are Pseudomonas eroginosa, Pseudomonas oryzihabita, Proteus varaplis, Klebsila pneumonia and Staphylococcus aureus to determine the most efficient solvent extraction of them, and then was use of a series of concentrations of the solvent is more efficient 0, 20, 40, 60, 80 and 100% to determine the most efficient concentration of solvent optimization, and then was determined the efficiency of minimum inhibitory concentration (MIC) of the extract.
Results: The results of the current study showed that the most efficient in the extraction solvent is acetone and the diameters of inhibition zone are 18, 19.83, 17.16, 16.33, 16.5 mm for the types of bacteria above, respectively. The results showed that the concentration of acetone 100% is the best concentration in extraction, amounting to the diameters of inhibition zone at this concentration of 17.33, 20, 19.33, 17.66,21.66 mm for each of the bacterial species above, respectively. The results also found that MIC is 6 mg/ml of Pseudomonas eroginosa and 7mg/ml of Pseudomonas oryzihabita and 11 mg/ml of each Proteus varaplis, Klebsila pneumonia and Staphylococcus aureus.
Conclusion: The most effective composites against pathogenic bacteria from Lawsonia inermis leaves are using acetone solvent with concentration of 100%.

The post Study of antibacterial activity of Lawsonia inermis leaf extract first appeared on Applied Medical Sciences.

Alaa Abdul-Hussein Al-Daamya, Ula Bahaaa, Dhuha Alaaddina, Marwa Hassana

Objectives: The present study aims to determine the inhibitory efficiency of Punica granatum peel extract and test its effectiveness against pathogenic bacteria.
Methods: Acetone, ethanol, methanol, ethyl acetate and distilled water were used as solvent to obtain of crude extract of P. granatum peel, which tested to determine the effectiveness against five types of pathogens, such as Pseudomonas aeruginosa, Pseudomonas oryzihabitans, Proteus vulgaris, Klebsiella pneumonia and Staphylococcus aureus under a series of concentrations. It was also used to determine the most efficient concentration of solvent optimization, and then was determined minimum inhibitory concentration (MIC) of the extract more efficient.
Results The results of the current study showed that the most efficient extraction solvent is ethyl acetate showed the diameters of inhibition zone are 48.66, 51.5, 41.66, 28.0, and 40.83 mm for the types of bacteria above, respectively. The results: showed that the concentration of ethyl acetate was 40% in the optimal inhibition of bacteria, amounting to diameters of inhibition zone at the concentration of 19.83, 27.33, 17.66,15.16 and 12.33 mm for each of the bacterial species above, respectively. The results also found that MIC is 11 mg/ml of P. aeruginosa and 10 mg/ml of P. varaplis and 9 mg/ml of P. oryzihabitans and 3 mg/ml of K. pneumoniae and S. aureus.
Conclusion: The most effective composites against pathogenic bacteria from P. granatum peel are using ethyl acetate solvent with the concentration of 40%.

The post Investigation of inhibition efficiency of Punica granatum peel extract against bacteria first appeared on Applied Medical Sciences.

Alaa Abdul-Hussein Al-Daamy, Haider Abd-Al Ameer, Hasan Zuher, Hussein Monather, Bashaer Ahmmed & Niesreen Kadhim

Objective: This study includes the investigation of antifungal activity of the local propolis against dermatophytes and yeast.
Methods: Two fungi belonging to dermatophytes, Trichophyton mentagrophytes and T. tonsurans, isolated from patients were tested. Six concentrations (0, 5, 10, 15, 20, and 25 mg/ml) of propolis extract were tested against these two fungal pathogens. Clotrimazole was used as control with 2 mg/ml concentration. So, five oral Candida albicans isolates were extracted from patients, these isolates were given numbers 1, 2, 3, 4 and 5. Seven concentrations (0.5, 1, 5, 10, 15, 20 and 25 mg/ml) of propolis extract were tested against C. albicans isolates.
Results: The results revealed presence of significant difference (P < 0.05) in the effect of propolis extract against two dermatophytes of the current study. T. tonsurans was more sensitive to propolis extract terms, the percentage of inhibition being 100% in each of the concentrations (10, 15, 20, 25 mg/ml), whereas it showed 72.4% at 5 mg/ml concentration. T. mentagrophytes did not show complete inhibition percentage which gave a high percentage, 94.2% with 25 mg/ml concentration. Additionally, results revealed presence of significant difference (P < 0.05) in the effect of propolis extract against C. albicans isolates of this study. C. albicans 1 isolate was the most effective isolate among the others toward propolis extract. Results of this study also showed increasing inhibition zone if the concentration of propolis extract was increased.
Conclusion: The local propolis that was collected from the apiaries of holy Karbala province has antifungal activity against T. mentagrophytes, T. tonsurans and C. albicans isolates.

The post Antifungal activity of propolis against dermatophytes and Candida albicans isolated from human mouth first appeared on Applied Medical Sciences.

Alaa A Kareem, Noor Y Abdzaid, Raghad M Salman, Murtadha K Mohamed, Ali J Dekel & Rawaa S Abdul-Muhsen

Objective

This study includes the investigation of antibacterial activity of the local propolis against four types of bacteria isolated from
patients.
Methods: Bacteria were tested including Psedomonas sp, Streptococcus sp, Escherichia coli and Staphylococcus aureus. Six concentrations (0, 5, 10, 15, 20 and 25) mg/ml of propolis extracts were tested against bacteria.
Results: Results revealed the presence of significant difference (P < 0.05) in the effect of propolis extract against the four types of bacteria in this study. Psedomonas sp. was the most sensitive among the others toward the propolis extract followed by Streptococcus sp., E. coli and Staphylococcus aureus at a rate of inhibition zones (14.09, 10.39, 8.78 and 8.39) mm, respectively. Results of this study also showed increasing rate of inhibition zone if the concentration of propolis extract was increased.
Conclusion: This study provided that local propolis has antibacterial activity against gram positive and gram negative bacteria.

The post Study of antibacterial activity in the local Iraqi propolis first appeared on Applied Medical Sciences.

ا.م.د مهدي عبد الخضر/جاكعة كربلاء ـ كلية الضيدلة

م.م.عبيرجياد يوسف/جامعة كربلاء ـ كلية العلوم

Abstract
This study condected to investigates of Mycobacterium tuberculosis from January till december 2007 from primary of care chest in Kirbala province , Atotal number collecting 339 (196 male &143 female).the study showed of infection with TB according to the months the higher percentage recorded in April then February reached (0.76,0.75) respectively. Also the affect of sex with infection disease showed high in male compared with female (0.55,0.43)respectively the study showed also
active the highest according to another type ofTB (0.85) the study showed relation between age and percentage of infection and found the redy of infection in the age under 34 years old.

The post Physiological and epidemiologic study for Mycobacterium tuberculosis in Kerbala Al Mukadssa first appeared on Applied Medical Sciences.

م.د مهدي عبد الخضر/جامعة كربلاء ـ كلية الصيدلة

م.م عبير جياد يوسف/جامعة كربلاء ـ كلية العلوم

Abstract
This study condected to perform on numbers of children with Diabetes mellitus in age between less than year to more than five year in Karbala and Najaf city for year (2010 – 2011 ), the Total number of state in Karbala for year 2010 was (805) , (435) for boys (370) for girls and the number of state for (2011 ) is ( 888) , ( 459) for boys,(429)for girls . while the number of state in Najaf for gear (2010) was ( 140) state , (78) for boys (62) for girls while the number of state to the same city
for year (2011) was (77) state (40) for boys and (37) for girls . The study showed no significant differences between sexual for two city in two years . but study showed significant differences(p≤0.01) in the age facter in all state and the highest state record in the age more than 5 year in Karbala for year 2011 reach to ( 612 . 67± 31.79) twen year 2010 ( 436.00± 17.32 ) in Najaf the highest state record also in the age more than 5 year in the year (2010) then (2011) , (19.00±5.19 ) and (47.00±2.30 ) respectively . also showed significant differences in Karbala for two year than Najaf city for child Diabetes infection

The post The study of some effect on happened rate of diabete mellite, in children in kerbala and Najaf(2010-2011) first appeared on Applied Medical Sciences.