The post The utility of human neutrophil peptide (1–3) in upper and lower pediatric urinary tract infections in kerbala province first appeared on Applied Medical Sciences.

The post INTERLEUKINS (4, 10, 18, 35) IMMUNOREGULATORY SYSTEMIC LUPUS ERYTHEMATOSUS IN KERBALA PROVINCE, IRAQ first appeared on Applied Medical Sciences.

The post Isolation and Characterization of Bacterial Infections from Patients with Prostste Cancer first appeared on Applied Medical Sciences.

Galangin Enhances Gold Nanoparticles as Anti-Tumor Agents
Against Ovarian Cancer Cells

Abstract. Galangin is a flavonoid from the root of Alpinia officinarum. It has novel anticancer properties. In the current
study, ovarian cancer cells SKOV3 were treated with galangin alone, galangin combined with Gold nanoparticles
(AuNPs) and AuNPs alone, and cytotoxic activity of these agentswas investigated using MTT viability assay. The
expression of apoptosis-related genes p53, caspase-8 was studied using Real time PCR assay. Galangin was tested at
different concentrations. AuNPs concentrations were 6.25,12.5, 25, 50 and 100 μg/ml. The study of gene expression
showed significant effects on p53, caspase8 in ovarian carcinoma cells. The results of the current study refer to galangin-
AuNPs combination is synergistic against to ovarian carcinoma to induce cytotoxicity and cell death via apoptosis, this
mechanism enhancing expression of p53, caspase-8. The effects of galangin, AuNPs,combination therapy have potential
clinicaluse in the future and could be alternative way to the conventional chemotherapy drug.

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The post Galangin Enhances Gold Nanoparticles as Anti-Tumor Agents Against Ovarian Cancer Cells first appeared on Applied Medical Sciences.

PHENOTYPIC AND GENOTYPIC DETECTION OF BIOFILM IN
STAPHYLOCOCCUS EPIDERMIDIS ISOLATED FROM SOME CLINICAL
SPECIMENS IN KERBALAPROVINCE

ABSTRACT : Staphylococcus epidermidis, the most frequently isolated coagulase-negative staphylococcus, is the leading
cause of infection related to implanted medical devices (IMDs). This is directly related to its capability to establish
multilayered, highly structured biofilm on artificial surfaces. At present, conventional systemic therapies using standard
antimicrobial agents represent the main strategy to treat and prevent medical device-associated infections. PCR assay
was used to detect the presence of icaA gene in the bacterial isolates, and the micro titter plate assay was used to detect
the ability for biofilm production. This study was aimed to detect the phenotypic and genotyping of virulence factors of
S.epidermidis bacteria isolated from patients with variety infections from different clinical specimens. As a result, the
biofilm production ability was strongly associated with the presence of icaA gene in the bacterial isolates.
Key words : Staphylococcus epidermidis, biofilm, coagulase negative Staphylococci, Nasocomial pathogen, ica operon.

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1r3buS731cFLV97tmuTU8AmCsEQWCZ2XD/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

The post PHENOTYPIC AND GENOTYPIC DETECTION OF BIOFILM IN STAPHYLOCOCCUS EPIDERMIDIS ISOLATED FROM SOME CLINICAL SPECIMENS IN KERBALAPROVINCE first appeared on Applied Medical Sciences.

EFFECT ANTI-SPERM ANTIBODY TYPE (IGG&IGA) ON
THE MACROSCOPIC AND MICROSCOPIC SPERM
EXAMINATION SPECIMEN FROM INFERTILE PATIENTS

Abstract:
Male infertility one of the most common health problems with psychological and social effects. This study aims to
investigate the effects of the antisperm antibodies(ASA) on the macroscopic and microscopic parameters of seminal
fluid in some infertile males.The percent of ASA type G (IgG), and (IgA) were measured on the surface of sperms,
macroscopic and microscopic examination of seminal fluid have been measured for infertile patient resorting to
Institute of Embryo Research and Infertility Treatment, Al-Nahrain University, and in the laboratories of Al-Hussain
Hospital /Kerbala. Throughout period then March to August 2011. (419)seminal fluid spaceman are collected from
infertile patient and measurement the percentage of (IgG&IgA) antisperm antibody to (38) sperm agglutination
specimen from infertile patient and comper with (12) fertile men as control group. The results were showed a
significant effect (P< 0.05) of IgG and IgA on a liquefaction time, percentage of progressive motility, sperm
agglutination, and round cells on compared with control group. These results showed a significant decrease (P<0.05)
in the percentage of rapped progressive motility (Grade A) in all groups.A significant increase (P<0.05) in sperm
agglutination and round cell in all groups. Positive correlation with sperm agglutination, morphologically abnormal
sperm percent and round cells. A significant increase (P<0.05) in liquefaction time in the second and the third group.
Key words:ASA,MAR test,IgA,IgG,seminal fluid

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1bai9BniFkFP6INMg8zrZEZzv5NhlTjmc/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

The post EFFECT ANTI-SPERM ANTIBODY TYPE (IGG&IGA) ON THE MACROSCOPIC AND MICROSCOPIC SPERM EXAMINATION SPECIMEN FROM INFERTILE PATIENTS first appeared on Applied Medical Sciences.

T-bet, Foxp3 and CD8 Regulating Immune Response in Colorectal Cancer

A B S T R A C T
Colorectal cancer (CRC) is the third most common cancer diagnosed, and is associated with high rates of propagation and
mortality for both men and women. Aimed to analyze impact of presence of T-bet, Foxp3 and CD8 on colorectal cancer.
During the period 1/October/2013 to 1/March/2014, fifty patients with colon cancer (14 female and 36 male) (7-75) years
were taken from (Al-Hussain Hospital City/Kerbala, Digestive and Liver Disease /Education Hospital Medical City Baghdad
and Teaching Oncology Hospital /Baghdad Medical City/ Baghdad /Iraq).Immunochemical studying of T-bet, Foxp3 and
CD8 of colorectal biopsies by using DAKO Company/Denmark. There was a significant increase (p≤0.001) in the
concentration of T-bet, Foxp3 and CD8 respectively in patients male compared with female, also there was a significant
variance (p≤0.001) in the three age groups from (7-20), (21-40) and (41-75) in the levels of T-bet and CD8, whereas a
significant difference (p≤0.05) in the concentration of Foxp3 in the three age groups. This study proved functional role of Tbet,
Foxp3 and CD8 in cell-mediated immunity.
Keywords: T-bet, Foxp3, CD8, Colorectal cancer.

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1iKuL8tyyAmZBTyjBdGw86Tux39KTIvQo/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

The post T-bet, Foxp3 and CD8 Regulating Immune Response in Colorectal Cancer first appeared on Applied Medical Sciences.

ASSESSMENT IMPORTANCE OF IL4, C3 AND C4 IN PATIENTS WITH SYSTEMIC LUPUS
ERYTHEMATOSUS

ABSTRACT
Systemic lupus erythematosus (SLE) is an autoimmune disease and a chronic systematic disease with unknown etiology influential more than one million
individuals each year. The aim of this study is to substantiation the impact of IL4, C3 and C4 in patients with Systemic Lupus Erythematosus. During the period
2/January/2013 to 1/October/2013, fifty patients with Systemic Lupus Erythematosus that diagnosed by clinic specialists at female with ages ranged between
(8-65) years, by using ANA indirect Immunofluorescence test, anti-dsDNA ELISA kit , C3,C4 Radial Immunodiffusion Plate , IL4 ELISA kit. There was an elevated levels
of the parameters; ANA, anti-dsDNA and IL-4 that measured in SLE patients which reflect the severity of disease comparing with controls group, also there was
importance of ANA, anti-dsDNA, C3, C4 and IL4 in the predictions of disease activity.
Keywords: ANA, anti-dsDNA, C3, C4, IL4 and SLE.

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1yDIdRdIe06eycrdFGJFD6W3wY-y-FRY2/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

The post ASSESSMENT IMPORTANCE OF IL4, C3 AND C4 IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS first appeared on Applied Medical Sciences.

ANTIBACTERIAL ACTIVITIES OF CINNAMON ZELANICUM SYZYGIUM AROMATICUM
ESSENTIAL OIL

ABSTRACT
Objective: Evalution the in vitro antibacterial activity of the essential oil isolated fromCinnamomum zeylanicum bark, Syzygium aromaticum
flowers, against Gram-positive (Staphyllococcus aureus, Streptococcus pyogenes) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa,
Proteus mirabilis, Serratia marcescens, Enterobacter cloacae, Klebsiella pneumonia ).

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1jjxpjvYSjrBl2gRG0iwzo1RYh3hiRseN/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

The post ANTIBACTERIAL ACTIVITIES OF CINNAMON ZELANICUM SYZYGIUM AROMATICUM ESSENTIAL OIL first appeared on Applied Medical Sciences.

Investigation of IL6, IL10, IFN-γ and TNF-α at Iraqi patients with colon cancer suffering type-2 diabetes

ABSTRACT
Objective: Diabetes mellitus is one of the risk factor for all cause of cancer such as colorectal cancer most common among females worldwide.
Aimed to investigate the serum levels of IL-6, IL-10 andIFN-γ, TNF-α in patients with colon cancer suffering diabetes mellitus.

[/mhc_text][mhc_button admin_label=”زر” button_url=”https://drive.google.com/file/d/1bLR2aXDPCT39vWyd8pawDQCmLiGCkIiS/view?usp=sharing” button_text=”Research” url_new_window=”off” button_style=”off” button_color=”#474747″ button_text_color=”#ffffff” background_layout=”dark” use_icon=”off” font_list=”mhicons” text_orientation=”right” animation=”off” button_fx=”off” button_size=”default” button_font=”off” wide_button=”off” /][/mhc_column][/mhc_row][/mhc_section]

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