Although aluminum is a ubiquitous metal in the ecosystem and has numerous critical roles in both the medicinal and biological fields, human daily life is seriously threatened by its assorted harmful influences. By this virtue, tracking the amount of aluminum by rapid sensitive and selective recognition methodologies is of great importance. Based on this, a novel fluorescent chemosensor 4,4′-(propane-2,2-diyl)bis(2-(((-2-hydroxybenzylidene) hydrazineylidene)-methyl)phenol) (BFASA) capable of recognizing Al3+ in a medium was constructed via an easy Schiff-base reaction between bisphenol A-containing molecule and the salicylaldehyde. The metal-binding studies of BFASA indicated a drastically enhanced emission with color alteration from colorless to green establishing the utility of BFASA against monitoring of Al3+ and only Cu2+/Al3+ significantly enhanced the absorbance intensity of the probe solution at 433 and 406, respectively. Its ability to selectively sense Al3+ demonstrated “switch-on” fluorescence responses for Al3+ with a low detection limit (LOD) of 0.56 μM and good selectivity, and pH adaptation range (5–8). The stoichiometric ratio of BFASA against the Al3+ was verified by the Job’s plot and TOF-MS analysis and determined as 1:2. To make the recognition process inexpensively, viable and straightforward, Smartphone application of BFASA was effectively applied to Al3+ sensing, which could benefit the on-site Al3+ recognition. In the fluorescence bio-imaging aspect, the BFASA could effectively monitor Al3+ in living cells.
