PHENOTYPIC AND GENOTYPIC DETECTION OF BIOFILM IN
STAPHYLOCOCCUS EPIDERMIDIS ISOLATED FROM SOME CLINICAL
SPECIMENS IN KERBALAPROVINCE
ABSTRACT : Staphylococcus epidermidis, the most frequently isolated coagulase-negative staphylococcus, is the leading
cause of infection related to implanted medical devices (IMDs). This is directly related to its capability to establish
multilayered, highly structured biofilm on artificial surfaces. At present, conventional systemic therapies using standard
antimicrobial agents represent the main strategy to treat and prevent medical device-associated infections. PCR assay
was used to detect the presence of icaA gene in the bacterial isolates, and the micro titter plate assay was used to detect
the ability for biofilm production. This study was aimed to detect the phenotypic and genotyping of virulence factors of
S.epidermidis bacteria isolated from patients with variety infections from different clinical specimens. As a result, the
biofilm production ability was strongly associated with the presence of icaA gene in the bacterial isolates.
Key words : Staphylococcus epidermidis, biofilm, coagulase negative Staphylococci, Nasocomial pathogen, ica operon.
