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OPTIMIZATION PRODUCTION CONDITIONS OF ANTIBACTERIAL METABOLITE FROM
STREPTOMYCES SP.

 

ABSTRACT
Objectives: The paper aimed to isolate Streptomyces strain having the ability to produce antibacterial metabolites and optimize some environmental
parameters for excellent antibiotic production.
Methods: Different soil samples were collected from extreme environments of desert regions at Karbala Province, Iraq. Actinomycetes were isolated
using different media. The primary screening for antibacterial production was accomplished, and the antibacterial activities were tested against
pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, and Pseudomonas aeruginosa. The most potent strain
was chosen for optimizing some of environmental parameters to increase the bioactive metabolite production. Different parameters were studied
such as culture media, temperature, pH, and agitation rate.

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