Under the Title of
(The Evaluation of the Efficiency of the Immunochromagraphic Strip Test Using the Polymerase Chain Reaction Technique (PCR) and other Methods Used in the Diagnosis of some Cute Diarrhea Causes in Pre-School Patients in the Governorate of Babil. A comparative Study)
An MSc Debate holds in AMS
In the Great Hall of Imam Al-Sadiq (PBUH), an MSc. thesis debate was held in the specialty of Medical Laboratories there. The thesis, written by the researcher (Zahraa’ H. Zebuun), titled as (The Evaluation of the Efficiency of the Immunochromagraphic Strip Test in Comparison with other Methods Used in the Diagnosis of some Diarrhea Causes in Pre-School Patients in the Governorate of Babil). The committee of debate was headed by Prof. Wefaa’ S. Shani PhD Uni. of Kerbelaa’, having its members: Prof. Hiyaam K. Al-Mas’uudi PhD Uni. of Babil and Asst. Prof. ‘Adil ‘A. Abid Ali PhD. Uni. of Kerbelaa’. The thesis was supervised by Prof. Hadi R. Hassan PhD -Uni. of Kerbelaa’. in addition to Asst. Prof. Haydar A. Muhammad PhD. Uni. of Kerbelaa’. .
The study aims at
Evaluating the efficiency of Immunochromagraphic Strip Test using the Polymerase Chain Reaction(PCR) so as to diagnose the Parasitic and viral reasons behind Acute Diarrhea, then, comparing it with the commonly used ones in present time.
Isolating and diagnosing some types of bacteria which cause the acute bacterial Diarrhea and
Evaluating them by using the PCR, and
Evaluating the sensitivity and the specificity of the methods used in such diagnosis.
The study demonstrates that the causes behind the acute Watery Diarrhea at the age of less than two years are primarily viral ones, then the bacterial ones come secondarily. On the other hand, the type of Diarrhea occurs due to the parasitic infection could be at the age between four and six years old mostly. The results manifested that the sensitivity and specificity of the Immunochromagraphic Strip Test which causes diarrhea is statistically very significant ones when being compared to the test of the PCR. Besides, the positivity of the test is probable for more than one parasite by the double and triple infections at the same time.
The study ends with some recommendations concerning:
the possibility of using the Immunochromagraphic Strip Test so as to diagnose the reasons behind acute diarrhea as a practical application in the laboratories of hospitals and private ones. It could be a substitution for the commonly used ones since it is an easy test which does not require a specialist in recognizing and diagnosing the parasites, having positive results of a highly sensitivity and specificity.
The possibility of using the Immunochromagraphic Strip Test to uncover and diagnose the causes once more in urine, saliva, Cerebrospinal Fluid (CSF) in addition to blood.
The use of this test to diagnose the parasites in the frozen samples of feces, considering it as a good criteria which enables the researchers to gather the samples primarily and then, do the test ; saving the researchers’ time and effort.
