Abstract:
Recently, dissemination of antibiotic resistant bacteria considered as a major public health concern. Therefore, bacteriophages gained great attention as a promising alternative therapy for resistant infectious diseases.
The current study aimed to isolate bacteriophages by simple methods and determining their efficiency against bacteria isolated from clinical samples.
Bacteria were first isolated from clinical specimens and identified using standard bacteriological and biochemical procedures. antibiogram of the isolated bacteria were determined using different antibiotic discs and the results were interpreted according to the clinical laboratory standards institute (CLSI) guidelines. Then sewage samples were processed using two protocols to isolate specific bacteriophages. Finally, antibacterial effect of bacteriophages was determined using Double Layer Agar(DLA) method.
The highest lytic activity of the isolated bacteriophages was seen using the first protocol. However, both methods showed antibacterial effect.
In conclusion, bacteriophages could be isolated using very simple methods.
Keyword: Bacteriophage, DLA, Multidrug resistant bacteria
